Abstract
The study of human endometrial-embryonic interactions is complicated by the destructive impact of endometrial sampling on the implantation process itself. Analysis of endometrial discharge is a novel technique that does not interfere with implantation. The primary objective of this prospective cohort study was to investigate whether a clinical pregnancy and implantation predictive cytokine profile can be identified in endometrial secretions aspirated immediately before embryo transfer after IVF. Endometrial secretions aspirated immediately prior to embryo transfer from 210 women undergoing IVF were analyzed by multiplex immunoassay for 17 soluble regulators of implantation, namely interleukin (IL)-1 beta, IL-5, IL-6, IL-10, IL-12, IL-15, IL-17, IL-18, tumor necrosis factor (TNF)-alpha, interferon (IFN)-gamma, macrophage migration inhibitory factor, eotaxin, protein 10 IFN-gamma inducible kDa (IP-10), monocyte chemoattractant protein-1 (MCP-1), dickhead homolog 1, heparin-binding epidermal growth factor, and vascular endothelial growth factor (VEGF). To detect implantation, daily urine samples were collected after embryo transfer and assayed for human chorionic gonadotropin (hCG) levels by immunoassay. Multivariate logistic regression analysis revealed significant associations (negative and positive association, respectively) between MCP-1 (p=0.005) and IP-10 (p=0.037) levels and the implant, and between IL-1 beta (p=0.037). =0.047) and TNF -alpha (P=0.023) levels and clinical pregnancy. The predictive value of IL-1 beta and TNF-alpha for pregnancy was found to be equivalent and additive to that of embryo quality. Cytokine profiling of endometrial secretion provides a novel and non-disruptive approach to study the role of the endometrium in human embryo implantation and identifies a profile that appears conducive to clinical pregnancy.
| Ursprache | Undefined/Unknown |
|---|---|
| Pages (from-to) | 1427-1435 |
| page number | 9 |
| diary | human reproduction |
| Volume | 24 |
| issue number | 6 |
| Of the | |
| release status | Published -2009 |
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Boomsma, CM., Kavelaars, A., Eijkemans, R., Lentjes, EG., Fauser, BCJM., Heijnen, CJ. y Macklon (2009).Endometrial secretion analysis identifies a predictive cytokine profile for IVF pregnancy.human reproduction,24(6), 1427-1435.https://doi.org/10.1093/humrep/dep011
Boomsma , CM ; Kavelaars, A.; Eijkemans, Rene et al. /Endometrial secretion analysis identifies a predictive cytokine profile for IVF pregnancy. In:human reproduction. 2009; Bd. 24, no. 6. S. 1427-1435.
@article{6090d7dc0ace4fe394264640e8455fe6,
title = "Analysis of endometrial secretion identifies a predictive cytokine profile for IVF pregnancy",
abstract="The study of interactions between the endometrium and the human embryo is complicated by the disruptive effects of endometrial sampling on the implantation process itself. Analysis of endometrial secretion is a novel technique that does not interfere with implantation. The primary objective of this prospective cohort study was to investigate whether a cytokine profile that predicts implantation and clinical pregnancy can be identified in endometrial secretions aspirated immediately before embryo transfer after IVF. Endometrial secretions aspirated immediately after IVF were analyzed. pre-embryo transfer from 210 women undergoing IVF using a multiplex immunoassay for 17 soluble regulators of implantation, namely interleukin (IL)-1 beta, IL-5, IL-6, IL-10, IL- 12, IL-15, IL-17, IL-18, tumor necrosis factor (TNF)-alpha, interferon (IFN)-gamma, macrophage migration inhibitory factor, eotaxin, IFN-gamma-inducible 10 kDa protein (IP-10), monocyte chemoattractant protein 1 (MCP-1), Dickkopf homologue 1, heparin-binding epidermal growth factor, and vascular endothelial growth factor (VEGF). To detect implantation, daily urine samples were collected after embryo transfer and assayed for human chorionic gonadotropin (hCG) levels by immunoassay. Multivariate logistic regression analysis revealed significant associations (negative and positive association, respectively) between MCP-1 (p=0.005) and IP-10 (p=0.037) levels and the implant, and between IL-1 beta (p=0.037). =0.047) and TNF -alpha (P=0.023) levels and clinical pregnancy. The predictive value of IL-1 beta and TNF-alpha for pregnancy was found to be equivalent and additive to that of embryo quality. Cytokine profiling of endometrial secretion provides a novel and non-disruptive approach to study the role of the endometrium in human embryo implantation and identifies a profile that appears to be conducive to clinical pregnancy.",
autor = "CM Boomsma y A. Kavelaars y Rene Eijkemans y EG Lentjes y Fauser, {BCJM (Bart)} y CJ Heijnen y Macklon",
year="2009",
doi = "10.1093/humrep/dep011",
language = "Undefined/Unknown",
volume = "24",
pages="1427--1435",
diary = "Human Reproduction",
issn = "0268-1161",
Publisher = "Oxford University Press",
number="6",
}
Boomsma, CM, Kavelaars, A, Eijkemans, R, Lentjes, EG, Fauser, BCJM, Heijnen, CJ y Macklon 2009, 'Endometrial secretion analysis identifies a predictive cytokine profile for IVF pregnancy',human reproduction, Bd. 24, no. 6, p. 1427-1435.https://doi.org/10.1093/humrep/dep011
Endometrial secretion analysis identifies a predictive cytokine profile for IVF pregnancy./Boomsma, CM; Kavelaars, A; Eijkemans, Rene et al.
In:human reproduction, vol. 24, no. 6, 2009, p. 1427-1435.
research result:contribution to the magazine›Article›Academic›Peer Review
YOU - LABEL
T1 - Endometrial secretion analysis identifies a predictive cytokine profile for IVF pregnancy
AU - Boomsma, CM
ES - Kavelaars, A
AU - Eijkemans, René
AU - Lentjes, EG
AU - Fauser, BCJM (Bart)
AU - Heijnen, CJ
AU - Macklon, null
PJ - 2009
J1-2009
N2 - The study of human endometrial-embryonic interactions is complicated by the destructive impact of endometrial sampling on the implantation process itself. Analysis of endometrial discharge is a novel technique that does not interfere with implantation. The primary objective of this prospective cohort study was to investigate whether a clinical pregnancy and implantation predictive cytokine profile can be identified in endometrial secretions aspirated immediately before embryo transfer after IVF. Endometrial secretions aspirated immediately prior to embryo transfer from 210 women undergoing IVF were analyzed by multiplex immunoassay for 17 soluble regulators of implantation, namely interleukin (IL)-1 beta, IL-5, IL-6, IL-10, IL-12, IL-15, IL-17, IL-18, tumor necrosis factor (TNF)-alpha, interferon (IFN)-gamma, macrophage migration inhibitory factor, eotaxin, protein 10 IFN-gamma inducible kDa (IP-10), monocyte chemoattractant protein-1 (MCP-1), dickhead homolog 1, heparin-binding epidermal growth factor, and vascular endothelial growth factor (VEGF). To detect implantation, daily urine samples were collected after embryo transfer and assayed for human chorionic gonadotropin (hCG) levels by immunoassay. Multivariate logistic regression analysis revealed significant associations (negative and positive association, respectively) between MCP-1 (p=0.005) and IP-10 (p=0.037) levels and the implant, and between IL-1 beta (p=0.037). =0.047) and TNF -alpha (P=0.023) levels and clinical pregnancy. The predictive value of IL-1 beta and TNF-alpha for pregnancy was found to be equivalent and additive to that of embryo quality. Cytokine profiling of endometrial secretion provides a novel and non-disruptive approach to study the role of the endometrium in human embryo implantation and identifies a profile that appears conducive to clinical pregnancy.
AB - The study of human endometrial-embryonic interactions is complicated by the destructive impact of endometrial sampling on the implantation process itself. Analysis of endometrial discharge is a novel technique that does not interfere with implantation. The primary objective of this prospective cohort study was to investigate whether a clinical pregnancy and implantation predictive cytokine profile can be identified in endometrial secretions aspirated immediately before embryo transfer after IVF. Endometrial secretions aspirated immediately prior to embryo transfer from 210 women undergoing IVF were analyzed by multiplex immunoassay for 17 soluble regulators of implantation, namely interleukin (IL)-1 beta, IL-5, IL-6, IL-10, IL-12, IL-15, IL-17, IL-18, tumor necrosis factor (TNF)-alpha, interferon (IFN)-gamma, macrophage migration inhibitory factor, eotaxin, protein 10 IFN-gamma inducible kDa (IP-10), monocyte chemoattractant protein-1 (MCP-1), dickhead homolog 1, heparin-binding epidermal growth factor, and vascular endothelial growth factor (VEGF). To detect implantation, daily urine samples were collected after embryo transfer and assayed for human chorionic gonadotropin (hCG) levels by immunoassay. Multivariate logistic regression analysis revealed significant associations (negative and positive association, respectively) between MCP-1 (p=0.005) and IP-10 (p=0.037) levels and the implant, and between IL-1 beta (p=0.037). =0.047) and TNF -alpha (P=0.023) levels and clinical pregnancy. The predictive value of IL-1 beta and TNF-alpha for pregnancy was found to be equivalent and additive to that of embryo quality. Cytokine profiling of endometrial secretion provides a novel and non-disruptive approach to study the role of the endometrium in human embryo implantation and identifies a profile that appears conducive to clinical pregnancy.
U2 - 10.1093/humrep/dep011
DO - 10.1093/humrep/dep011
M3 - artillery
Serial number - 0268-1161
VL-24
SP-1427
EP-1435
JO - Human Reproduction
JF - Human Reproduction
ES - 6
ES -
Boomsma CM, Kavelaars A, Eijkemans R, Lentjes EG, Fauser BCJM, Heijnen CJ et al.Endometrial secretion analysis identifies a predictive cytokine profile for IVF pregnancy.human reproduction. 2009;24(6):1427-1435. dos: 10.1093/humrep/dep011